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Endless Space 2 Gene Splicing

Endless Space 2 Gene Splicing >>

In this work, the purpose of this strategy was to discover a new splicing transcript and to elucidate its biological significance. For this purpose, we searched for intron-exon splicing junctions by using 454 sequencing technology, which enables the sequencing of full-length cDNA fragments, increasing the detection power of RNA editing events. Thus, full-length cDNA sequences of the branched aromatic amino acid aminotransferase (B-AAT) gene, containing specific introns present in the PfBAA2D transcript, were obtained and their splicing events, including novel splicing isoforms, were confirmed by nucleotide or amino acid sequence alignment. Technologically, nucleotide or amino acid sequence alignment of the divergent B-AAT gene alleles in parasite isolates was performed by using DNASTAR aligner software. Furthermore, the chromosomal locations of the corresponding genes and transcripts were obtained by PCR with a minimum overlap of the respective introns relative to the introns of the canonical B-AAT transcript PfBAA2D, using plasmodium genomic DNA. The proteins encoded by the new B-AAT transcripts were analyzed by protein sequence alignment and domain prediction. Altogether, the results showed that the B-AAT genes have been subjected to a complex process of reorganization with a series of small additions, a few deletions and, in some cases, alternative splicing, simplifying the gene structure and producing proteins with increased genetic complexity. These events were also confirmed in in silico analyses with arequip's "Kalign" software.

In this work, the intention was to infer variability of the P falciparum apicoplast organelle genome during the intraerythrocytic development of the parasite. The apicoplast genome of P. falciparum is a circular DNA molecule that shares homology with the genome of the eubacterial photosynthetic molecule, chloroplast. However, the chloroplast genome encodes only few proteins, whereas P. falciparum has within its genome about 40 copies of genes encoding proteins responsible for essential metabolic pathways. d2c66b5586


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